In vitro evaluation of (Trichoderma harzianum) for virulence efficacy on (Ganoderma lucidum)

Abstract

Nikita Nawani, Ashwani Tapwal

Antimycotic activity of Trichoderma harzianum to inhibit the growth of Ganoderma lucidum was evaluated under laboratory conditions. In the dual cultures, 20.18% growth inhibition was recorded on the fifth day of incubation. The volatile compounds released by T. harzianum have supressed the growth of G. lucidum in the range of 21.79%- 56.15% T. harzianum released non-volatile metabolites into the liquid media, which inhibited the growth of G. lucidum upto 34.13±0.05 percent. The rate of growth inhibition increased with the increase in the age of antagonist and concentration of culture filtrate in media. Trichoderma harzianum contained phytochemicals such as alkaloids, phenolics, tannins, flavonoids, carbohydrates, reducing sugar and proteins, but terpenoids and amino acids were not detected.