Zuluqrnain Khan
Institute of Plant Breeding and Biotechnology, MNS University of Agriculture, Multan, PakistanPublications
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Research
Assessment of Cry1Ac and Cry2A expression endotoxins in transgenic cotton across growth periods and stages under semi-controlled conditions
Author(s): Mahreen Hanif, Shafqat Saeed*, Zuluqrnain Khan, Mudssar Ali and Muhammad Ishtiaq
Transgenic cotton has brought about a significant transformation in the management of bollworms. Nevertheless, a considerable challenge has emerged in the form of Pectinophora gossypiellafor transgenic cotton. In current study, the detection and quantification of Cry1Ac and Cry2Ab protein in pot samples of different transgenic cotton cultivars encompassing single (Cry1Ac) and double gene (Cry2Ab) cultivars was conducted, employing bt strips and ELISA methods at three distinct time points, namely 40 days, 80 days, and 120 days after sowing, coinciding with different growth phases (i.e., leaves, squares, bolls, and seeds). The outcomes revealed that the highest toxin levels of Cry1Ac protein were present across all cultivars, whereas elevated levels of Cry2Ab protein were exclusively observed in MNH-1045 cultivar ranged from 0.05 ug/g to 1.46 ug/g. The findings highlighted visible diffe.. Read More»
DOI: 10.5281/zenodo.15093541