Nuha A. Alkhattabi
Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaPublications
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Research Article
The anti-inflammatory effect of Artemisia absinthium extract on cell growth through targeting PI3K/Akt/mTOR pathway in human hepatocellular carcinoma cell line
Author(s): Nuha A. Alkhattabi* and Nesrin I. Tarbiah*
Objective: Artemisia absinthium’s biological activity has been investigated for possible therapeutic applications. It is a plant that may be used in cancer treatment. The biochemical and molecular mechanisms of Artemisia absinthium Extract (AAE) treatment in the hepatic cancer HepG-2 cell line were investigated in this work. Method: Phytochemical profiling was performed using quantitative methods and GC-MS analysis to assess phytoconstituents in AAE. The in vitro cytotoxic effect of AAE on HepG-2 cellular viability was determined to assess the inhibitory concentrations that kill 50% of cells (IC50, μg/ml). Apoptosis and cell cycle arrest were investigated using flow cytometry analysis. Furthermore, gene expression levels of PI3K/Akt/mTOR genes as well as their protein levels in the AAE-treated HepG-2 and untreated cells, were evalua.. Read More»
DOI: 10.5281/zenodo.16809969