Givin J. Hosburgh
Department of Animal and Plant Sciences, University of Sheffield, Sheffield, UKPublications
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Research Article
Molecular characterization of genetic diversity and mycorrhizal responsiveness in maize (Zea mays) cultivars using 18s rRNA, RBCL and ISSR markers
Author(s): Amal Y. Aldhebiani*, Amal T. K. Ashour, Neveen S. Geweely, Mohamed Abu-Zeid, Givin J. Hosburgh, Duncan D. Cameron and Salah E. M. Abo-Aba
Maize (Zea mays) is a globally significant crop, and its genetic diversity is crucial for its adaptation and improvement through breeding programs. This study employed molecular markers 18S rRNA, RBCL gene sequences, and Inter-Simple Sequence Repeats (ISSR) to assess the genetic diversity and mycorrhizal responsiveness of 18 maize cultivars cultivated in Saudi Arabia. Phylogenetic analysis using maximum likelihood methods revealed two major clades, with significant variation in mycorrhizal responsiveness among genetically similar cultivars. The results indicate that while genetic relatedness is a factor, it is not a strong predictor of mycorrhizal symbiosis. ISSR markers were particularly effective in revealing polymorphisms, with the cultivars displaying considerable genetic diversity, thus supporting their use in future marker-assisted selection and breeding programs. These.. Read More»
DOI: 10.5281/zenodo.200121