Faiz MMT Marikar
Department of Health Science and Education, The Open University of Sri Lanka, 10350, Sri LankaPublications
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Research
An efficient plant regeneration, detection and identification of secondary metabolites from propagate plants of Peperomia pellucida (L.) for mass cultivatione
Author(s): Abdul Bakrudeen Ali Ahmed, Teoh Lydia, Muneeb Muhamed Musthafa, Rosna Mat Taha and Faiz MMT Marikar
Several species in the genus Peperomia Ruiz and Pav have giant chloroplasts in the palisade parenchyma of their leaves. Because of this unique feature, Peperomia pellucida L. has become a valuable model plant for studying plastid biology. However, the use of Peperomia for molecular studies has been limited by the lack of efficient regeneration and transformation protocols. This study describes an effective protocol for in vitro shoot induction, plant regeneration and rooting from internode of explant of Peperomia pellucida using Murashige and Skoog (MS) medium supplemented with different plant growth regulators. The multiple shoots were induced from internodes cultured on MS medium containing Kinetin (KN) (0.5 mg L-1) alone which induced six shoots per internodal explant. In combination Benzyladenine (BA) (1.5 mg L-1) and KN (1.5 mg L-1.. Read More»
DOI: 10.5281/zenodo.5078526